![]() ![]() Conformations of the third hypervariable region in the VH domain of immunoglobulins. Morea, V., Tramontano, A., Rustici, M., Chothia, C. High-throughput immune repertoire analysis with IGoR. High-resolution description of antibody heavy-chain repertoires in humans. In-depth determination and analysis of the human paired heavy- and light-chain antibody repertoire. Estimating the richness of a population when the maximum number of classes is fixed: a nonparametric solution to an archaeological problem. Nonparametric Estimation and Comparison of Species Richness (John Wiley & Sons, 2016).Įren, M. Robust estimates of overall immune-repertoire diversity from high-throughput measurements on samples. Estimating the population size for capture–recapture data with unequal catchability. Multi-donor longitudinal antibody repertoire sequencing reveals the existence of public antibody clonotypes in HIV-1 infection. Measurement of ‘overlap’ in comparative ecological studies. ![]() Measuring of the dispersion of individuals and analysis of the distributional patterns. Reference values for B cell subpopulations from infancy to adulthood. Clonify: unseeded antibody lineage assignment from next-generation sequencing data. ![]() Massively scalable genetic analysis of antibody repertoires. Deep sequencing and human antibody repertoire analysis. The Generation of Antibody Diversity (Garland Science, New York, 2002).īoyd, S. Clonal selection and learning in the antibody system. This dataset enables genetic study of the baseline human antibody repertoire at an unprecedented depth and granularity, which reveals largely unique repertoires for each individual studied, a subpopulation of universally shared antibody clonotypes, and an exceptional overall diversity of the antibody repertoire. Here we examine the circulating B cell populations of ten human subjects and present what is, to our knowledge, the largest single collection of adaptive immune receptor sequences described to date, comprising almost 3 billion antibody heavy-chain sequences. Furthermore, because much of the B lymphocyte population is localized in organs or tissues that cannot be comprehensively sampled from living subjects, human repertoire studies have focused on circulating B cells 3. The amount of information encoded by all of the rearranged antibody and T cell receptor genes in one person-the ‘genome’ of the adaptive immune system-exceeds the size of the human genome by more than four orders of magnitude. ![]() Full-scale analyses of human antibody repertoires have been prohibitively difficult, primarily owing to their massive size. Because the number of peripheral blood B cells in a healthy adult human is on the order of 5 × 10 9, the circulating B cell population samples only a small fraction of this diversity. The diversity of the naive antibody repertoire in humans is estimated to be at least 10 12 unique antibodies 2. This flexibility is achieved by the presence of a large repertoire of naive antibodies, the diversity of which is expanded by somatic hypermutation following antigen exposure 1. In principle, humans can produce an antibody response to any non-self-antigen molecule in the appropriate context. ![]()
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